In order to obtain a good separation and resolution of MFA and MFB in the chromatograms, various compositions of mobile phasa (such as methanol-H2O (v/): 60:40,70:30,80:2090:10 and acetontrile-H2O (v/v): 60:40,70:30,80:20,90:10) were tried to obtain chromatogtams with good resolution of adjacent peaks.