The apparent ileal and excreta digestibilities of amino acids in 15 samples representing 12
food ingredients were determined using 5-week-old male broiler chickens. The ingredients included 3
samples of cereals (wheat, maize and sorghum), 6 samples of plant protein meals (soyabean meal, cottonseed
meal, canola meal and sunflower meal) and 6 samples of animal protein meals (meat meal, meat-andbone
meal, feather meal and fish meal).
2. The test ingredients were incorporated as the sole source of dietary protein in assay diets. Each diet was
offered ad libitum to 3 pens (4 birds/pen) from d 35 to d 42 post-hatching. Total collection of excreta was
carried out during the last 4 d. All birds were killed on d 42 and the contents of the lower half of the ileum
were collected. Apparent ileal and excreta amino acid digestibilities were calculated using acid-insoluble ash
as the indigestible marker.
3. The influence of site of measurement was found to vary among food ingredients, among samples within
an ingredient and among different amino acids within an ingredient. Ileal amino acid digestibility values
were similar in some ingredients, but significantly lower or higher in others than the corresponding excreta
values.
4. Average ileal and excreta amino acid digestibilities in sorghum and maize were similar, but significant
differences were observed for individual amino acids. In contrast, ileal amino acid digestibility values were
higher than the corresponding excreta digestibility values in wheat.
5. The average ileal and excreta digestibilites of amino acids in the 3 soyabean meal samples were similar
although small, but significant differences were noted for individual amino acids. Site of measurement had
no effect on the digestibility of amino acids in canola meal. Digestibilities of valine, isoleucine, phenylalanine,
histidine, glutamic acid, alanine and tyrosine in sunflower meal and those of valine, methionine,
isoleucine, leucine, lysine, glutamic acid and alanine in cottonseed meal were lower by excreta analysis.
6. Digestibilities in animal protein meals, with the exception of blood meal and fish meal, were consistently
higher by excreta analysis. Ileal-excreta differences in individual amino acid digestibilities were more evident
in feather meal, meat meal and meat-and-bone meal.
7. Threonine and valine were the indispensable amino acids that were more frequently influenced by the
site of measurement. Of the dispensable amino acids, aspartic acid, serine, glutamic acid and alanine were
the most affected.
8. Differences determined between ileal and excreta digestibilities in the present study clearly demonstrate
that amino acid metabolism by hindgut microflora in chickens may be substantial and that digestibilities
measured in the terminal ileum are more accurate measures of amino acid availability than those measured
in the excreta.
The apparent ileal and excreta digestibilities of amino acids in 15 samples representing 12food ingredients were determined using 5-week-old male broiler chickens. The ingredients included 3samples of cereals (wheat, maize and sorghum), 6 samples of plant protein meals (soyabean meal, cottonseedmeal, canola meal and sunflower meal) and 6 samples of animal protein meals (meat meal, meat-andbonemeal, feather meal and fish meal).2. The test ingredients were incorporated as the sole source of dietary protein in assay diets. Each diet wasoffered ad libitum to 3 pens (4 birds/pen) from d 35 to d 42 post-hatching. Total collection of excreta wascarried out during the last 4 d. All birds were killed on d 42 and the contents of the lower half of the ileumwere collected. Apparent ileal and excreta amino acid digestibilities were calculated using acid-insoluble ashas the indigestible marker.3. The influence of site of measurement was found to vary among food ingredients, among samples withinan ingredient and among different amino acids within an ingredient. Ileal amino acid digestibility valueswere similar in some ingredients, but significantly lower or higher in others than the corresponding excretavalues.4. Average ileal and excreta amino acid digestibilities in sorghum and maize were similar, but significantdifferences were observed for individual amino acids. In contrast, ileal amino acid digestibility values werehigher than the corresponding excreta digestibility values in wheat.5. The average ileal and excreta digestibilites of amino acids in the 3 soyabean meal samples were similaralthough small, but significant differences were noted for individual amino acids. Site of measurement hadno effect on the digestibility of amino acids in canola meal. Digestibilities of valine, isoleucine, phenylalanine,histidine, glutamic acid, alanine and tyrosine in sunflower meal and those of valine, methionine,isoleucine, leucine, lysine, glutamic acid and alanine in cottonseed meal were lower by excreta analysis.6. Digestibilities in animal protein meals, with the exception of blood meal and fish meal, were consistentlyhigher by excreta analysis. Ileal-excreta differences in individual amino acid digestibilities were more evidentin feather meal, meat meal and meat-and-bone meal.7. Threonine and valine were the indispensable amino acids that were more frequently influenced by thesite of measurement. Of the dispensable amino acids, aspartic acid, serine, glutamic acid and alanine werethe most affected.8. Differences determined between ileal and excreta digestibilities in the present study clearly demonstratethat amino acid metabolism by hindgut microflora in chickens may be substantial and that digestibilitiesmeasured in the terminal ileum are more accurate measures of amino acid availability than those measuredin the excreta.
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