Exposure of ammonia among workers from fresh natural latex purchasing process was risked to abnormal functions of pulmonary causing from inside and outside personnel factors. The aim of this study was to determine factors associated to the abnormal of pulmonary functions of fresh natural latex purchasing workers at Rubber Holder Cooperative in Southern Thailand. Questionnaires, spirometer, tape measure, digital balance, and ammonia measure were used for collecting the information. Those information were compared between 55 workers and other 55 regularly rubber farmers. The result found that an abnormal pulmonary function was occurred in the fresh natural latex purchasing workers and rubber farmers at 9.1% and 1.8%, respectively. Different factors were studied consisting of gender, status, education level, experience of working, the using of personal protective equipment (PPE), smoking status, chronic cough, and body mass index (BMI). The result found that the luck of using PPE associated to the pulmonary functions (OR 9.9, 95% CI 1.6- 67.6).Therefore, the relating network of partnership organization have to provide the respiratory PPE to the worker and give them the correctly and properly of knowledge, instruction including the maintenance guideline of an equipment.