Fatty acid profile with GC–FID
The analysis of the fatty acid methyl ester profiles of SIO and
mixtures with SO and CO were performed using a Hewlett Packard
HP 5890 – Series II, (Palo Alto, CA, USA) gas chromatograph,
equipped with a flame ionization detector (GC–FID) and a
BPX70-ms (Thermo Scientific, Waltham, MA, USA) capillary column
(30 m 0.25 mm 0.25 lm) composed of 70% cyanopropylpolysilphenylene-siloxane.
The initial temperature was 150 C/
min, which increased by 5 C/min up to 240 C and was held constant
for 30 min. The injector temperature was 240 C and the
detector temperature was 280 C, with a split ratio of 1:30. The carrier
gas used was He at 1 mL/min, at a pressure of 11 psi.