Foliar spraying treatment on wheat seedlings
The plant extracts were tested for their efficacy in controlling
leaf rust using foliar spray application on wheat seedlings
in the greenhouse. For each treatment, grains of the highly susceptible
wheat variety Morocco were sown in pots (15 cm
diameter) with three replicates (10–15 grains/pot). Sumi-8 fungicide
was used as standard treatment (positive control).
Seedlings sprayed with water supplemented with four drops
of Tween 20 were served as negative control (untreated control).
All extracts were separately sprayed one day before inoculation
with uredospores and one and four days after inoculation
with uredospores. Inoculation and incubation were performed
as described by Ref. [25]. After 15 days from inoculation,
disease severity was rated in terms of infection types and
number of pustules/leaf was recorded.