To increase the production of
PHA, 3-L stirred bioreactor with 1-L working volume was
considered using the optimal condition obtained from the
Erlenmeyer flask scale (1.5% v/v linoleic acid as sole carbon
source). The CDW and PHA production as function of time
are shown in Figure 4. The batch system reached a steady
state with the CDW (6.75 g/L) and the maximum PHA (3.05
g/L) after 48 h, representing 45.2 wt% of accumulation.