Conclusions
Presence of nisin in edible films formulated with tapioca
starch and glycerol reduced L. innocua growth, producing
count decrease and acting as a barrier to contamination
after processing.
The study of nisin performance through the agar diffusion
technique was not satisfactory due to the competence
between rate of nisin diffusion, diameter increase due to
swelling and microbial growth. However, studied bacteria
showed a decreased population under the film with nisin
which also produced clear inhibitory zones.
The initial release of nisin to the liquid medium produced
an important inhibition of L. innocua, specially when