2.11. Hydrolysis of phytate by S. cerevisiae LC3 under simulated GI conditions
Whole wheat bread, fermented by commercial baker's yeast and L. plantarum LP41 and containing 5.31 mg/g of phytate was used. Briefly, 100 mg of bread were suspended in 4 mL of 0.2 mol/L HCl.
(pH 2.0) and homogenized in a Sterilmixer Lab (PBI International). This suspension was added of ca. 9.0 log CFU/g of S. cerevisiae LC3 and subjected to pepsin and pancreatin digestion as described in the Section 2.10 (Pasini et al., 2001). After digestion, the suspension was further incubated for 24 h at 37 C, under stirring conditions, to mime the hydrolysis of phytate by yeasts colonizing the GI tract. Digested samples were recovered after 2, 4 , 16 and 24 h of incubation and analysed for the concentration of phytate as described in the Section 2.8. All treatments were also carried out using whole wheat bread without the addition of LC3
( control ).