Abstract:-Plant-based medicines play an important role in all cultures, and have been
indispensable in maintaining health and combating diseases. Owing to the global trend
towards improved quality of life, there is a great demand for medicinal plants. Various parts of
this plant have long been used in traditional system of medicine. Hence the present study has
been conducted in the light of immense potential of medicinal plants used in various traditional
systems. The purpose of the study was to determine the different phytochemical compounds of
leaf extracts of Carissa carandas, their antioxidant properties, antimicrobial activities and
cytotoxic potentials. Here, ethanolic and n-hexane extracts of the leaves of Carissa carandas
were used. Results showed significant antioxidant activities compared to ascorbic acid and
BHT in DPPH free radical scavenging with IC50 of 1.292 μg/ml and 1.824 μg/ml of ethanolic
extract and n-hexane extract. H2O2 scavenging activities of the extracts were found to be better
than the standard, having IC₅₀ values higher than ascorbic acid. Total antioxidant activity and
total phenolic content were also determined. Leaf extracts showed no antimicrobial activity
through disk diffusion method using extracts ranging from 0.1- 400 μg/disc when compared to
Kanamycin disc of 30 μg/disc. Cytotoxic activities of the extractives were comparable to
vincristine sulfate, having IC50 values of 2.818 and 1.995 of ethanolic extract and n-hexane
extract respectively. The present study provided data justifying the use of this plant for
medicinal purposes.