Total vitamin C (ascorbic acid + dehydroascorbic acid) has been determined by UV- spectrophotometric method in various fruits and vegetables. In this method bromine water oxidizes ascorbic acid to dehydroascorbic acid in presence of acetic acid. After coupling with 2,4-dinitrophenyl hydrazine at 37OC temperature for three hours, the solution is treated with 85% H2SO4 to produce a red color complex and the absorbance was spectrophotometrically measured at 521 nm. The content of vitamin C were 10 mg/100g to 80 mg/100g in fruits and 16 mg/100g to 42 mg/100g in veg- etables. A loss of 64% at -10OC and 76% at 5OC was observed after two months stor- age period of one leafy vegetable "Enhydra fluctuans" (Helencha shak).