Thirteen (4.8%) cats were positive to D. immitis, while antibodies to L. infantum were detected in 10 (3.7%) animals.The prevalence of D. immitis and L. infantum in the feline population from southern Portugal should alert for the need to implement control measures to protect animals and people from these zoonotic parasites. Furthermore, both parasitoses must be included in the differential diagnosis in feline clinical practice.