The homogenate was centrifuged at 10,000 g for 15 min.The supernatant was used for enzyme assays and all of the extraction steps were carried out at 1–4 8C (Trevor and Fletcher, 1994).
The homogenate was centrifuged at 10,000 g for 15 min.The supernatantwas used for enzyme assays and all of the extraction steps werecarried out at 1–4 8C (Trevor and Fletcher, 1994).