The selective bioassays were performed on fresh artificial diet, comprising 10 g agar (Biobra´s, Brazil), 15.6 g brewer’s yeast, 25 g wheat germ, 25 g soy protein 31.2 g minced beans, 2.2 g ascorbic acid, 12.5 g casein, 2.5 ml vitamin solution (1.2% ascorbic acid, 0.03% calcium pantothenate, 0.015% niacin, 0.008% riboflavin, 0.004% Thiamin HCl, 0.004% pyridoxine HCl) in a total volume of 1.0 l poured into 50 ml plastic cups (5 ml per cup).