the induction of callus formation. Likewise, Swamy et al. (2010)
reported a decrease in shoot production in Pogostemon cablin at
higher concentrations of BA. Stunted shoots that developed in the
medium corresponding to increased concentrations of BA have
been reported for Orthosiphon stamineus (Lai-Keng and Leng, 2004)
and in the same manner as Eupatorium triplinerve (Martin, 2004). It
was also found that calli that were grown on MS media containing
3 mg/L BA and 0.3 mg/L IAA were able to develop 5.25 ± 0.73 shoots
per explant (±SE).
In addition, BA was also observed to affect the height of P. helferi,
with MS media containing IAA without BA generating the tallest
plants as shown in Table 5. The results showed that the stems and
leaves were noticeably larger in the MS medium with 0.3 mg/L IAA
added (Fig. 2C). Effective regeneration and healthy shoot development
were also observed in these treatments as the plant growth
regulator IAA affected the expansion of plant cells (Skoog and
Miller, 1957)