3.2. Chromatogram of C. asiatica (雷公根lei gong gen) extracts in
high performance liquid chromatography (HPLC)
We calculated the content in CA extracts with HPLC. After
comparing the chromatogram of CA extracts with four standard
solutions, including madecoside (MS), asiaticoside (AS), madecassic
acid (MA) and asiatic acid (AA), we confirmed that MS and AS were
present, but not MA and AA. After quantitative analysis, MS content
accounted for 1.20% of the CA extracts, while AS content accounted
for only 0.17%. Because the most effective dose in CA extracts was
50 mg/mL, we used this concentration to calculate the most effective
concentration of MS and AS. Using this value, the concentration of
MS was found to be 6.0 mg/mL, calculated as 50 mg/mL 1.20%,
while the concentration of ASwas 0.085 mg/mL, calculated as 50 mg/
mL 0.17% (Fig. 2A and B).