In the clinical context, the diazo-reaction is most
often used and the determination of TB and DB will
allow defining the hyperbilirubinaemia as:
1. Unconjugated hyperbilirubinaemia: DB/
TBo20–30%. In this condition, one has to consider:
Haemolysis: Characterized by a high reticulocyte
count, low free haptoglobin, high serum iron and
LDH. Erythrocyte abnormalities may be recognized
in blood smears. Splenomegaly is often detectable.