Rehydrate the contents of one vial aseptically with 3 ml sterile distilled water. Add 0.5 ml of rehydrated FD248 in a tube. To
this add approximately 0.05 ml of overnight broth culture of test organisms or 2-3 pure colonies picked from a non-inhibitory
Agar plate. Mix gently & incubate at 37°C in incubator or water bath for up to 4 hours. Observe for clot formation in the tube
at regular intervals. Any degree of clotting within 4 hours is considered as positive results.