Materials and methods
Cattle
Cross-bred (Bos indicus9Bos taurus) bovine calves (n = 9)
were procured from Livestock Production and Management
section of the Institute, Izatnagar just after weaning and
maintained in the tick proof shed of the Division of Parasitology.
The calves were fed on a daily ration of concentrate
and wheat bhusa with ad lib drinking water. Each calf was
supplemented with vitamin A and mineral mixture at regular
interval. The experimental animals were maintained
according to the guidelines of Committee for the Purpose of
Control and Supervision of Experimentation on Animals
(CPCSEA), a statutory Indian body.