light-limiting conditions, the high productivity
reached outdoor under high irradiances indicates
that this productivity could be yet enhanced, thus
P. cruentum being a potential source of these
products. In addition, P. cruentum had both types
of phycobiliproteins; phycoerythrin and phycocyanin.
However, there was only a compound for
each type of phycobiliprotein, B-PE and R-PC,
thus making easier the downstream process.
Until now very different methodologies have
been proposed for purifying phycobiliproteins
from microalgae but only some of them are useful
for scale-up. In addition, the yield of the proposed
process is usually not calculated only focusing on
the purity of the product. Most of the proposed
methods could be divided into two steps: a first
step of extraction of the phycobiliproteins from
the biomass, and a second step of purification of
the obtained extract. In our work, evaluating the
yield of each step in the initial stages (Fig. 7) it
was observed that mainly phycobiliproteins were
released in the extraction (step 1) and precipitation
(step 3). Thus, in the extraction process (step
1) only 60% of the phycobiliproteins were recovered,
whereas in the precipitation process (step 3)
the yield was 75%. The overall recovery at the end
of the pre-treatments was only 43%. In addition,
the yields were slightly different for the two phycobiliproteins,
being higher for B-PE (45.4% recovery)
and lower for R-PC (33.0% recovery).
Thus, the yield of the initial stages needs to be
enhanced. There are different possibilities, from
optimisation of the cell disruption, extraction and
precipitation conditions, to use of other methodologies
such as expanded bed chromatography or
ultrafiltration. Anywhere, the methodology used
in this work provides better results than others
referenced. Thus, Galland-Irmouli et al. (2000)
Fig.
light-limiting conditions, the high productivity
reached outdoor under high irradiances indicates
that this productivity could be yet enhanced, thus
P. cruentum being a potential source of these
products. In addition, P. cruentum had both types
of phycobiliproteins; phycoerythrin and phycocyanin.
However, there was only a compound for
each type of phycobiliprotein, B-PE and R-PC,
thus making easier the downstream process.
Until now very different methodologies have
been proposed for purifying phycobiliproteins
from microalgae but only some of them are useful
for scale-up. In addition, the yield of the proposed
process is usually not calculated only focusing on
the purity of the product. Most of the proposed
methods could be divided into two steps: a first
step of extraction of the phycobiliproteins from
the biomass, and a second step of purification of
the obtained extract. In our work, evaluating the
yield of each step in the initial stages (Fig. 7) it
was observed that mainly phycobiliproteins were
released in the extraction (step 1) and precipitation
(step 3). Thus, in the extraction process (step
1) only 60% of the phycobiliproteins were recovered,
whereas in the precipitation process (step 3)
the yield was 75%. The overall recovery at the end
of the pre-treatments was only 43%. In addition,
the yields were slightly different for the two phycobiliproteins,
being higher for B-PE (45.4% recovery)
and lower for R-PC (33.0% recovery).
Thus, the yield of the initial stages needs to be
enhanced. There are different possibilities, from
optimisation of the cell disruption, extraction and
precipitation conditions, to use of other methodologies
such as expanded bed chromatography or
ultrafiltration. Anywhere, the methodology used
in this work provides better results than others
referenced. Thus, Galland-Irmouli et al. (2000)
Fig.
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