The study of theoretical
specificity corresponded to the choice of a region that makes the
difference between Atlantic salmon (S. salar L.) and all other species
of salmonids. The alignment allowed us to choose the adequate
positions of the primers and probe for the differentiation
between the trout (Salmo trutta) and the salmon S. salar GH1 and
thus avoiding false positive results in further amplifications with
real-time PCR. The results of the sequence alignment and the primer
and probe locations are shown in Fig. 1.