Standard solutions of commercially
available capsaicin were prepared and subsequently used
to prepare an HPLC calibration curve of total peak area
and the sum of the peak areas for capsaicin and
dihydrocapsaicin gave the total concentration of the two
major capsaicinoids. The peaks eluting prior to 4.5 min on
this chromatogram are carotenoids and other pigment
materials in the sample. In the chromatogram, both
capsaicin and dihydrocapsaicin give strong and wellresolved
peaks. Among the two test solvents used in this
study, acetonitrile showed less pigments with high
capsaicin content when compared to the acetone. At the
time interval of 4.5 min the pure capsaicin can be eluted
for commercial applications. According to Joseph
Amruthraj33 the acetonitrile extracts of C. chinense fruits
showed wide antibacterial activities against the human
pathogens and concluded that capsaicin present in C.
chinense fruits is very effective in the prevention of many
diseases.