and they have high carbohydrate contents. In this facility, CHEMEX pretreatment with sodium hydroxide was applied to improve the enzymatic digestibility. After pretreatment, the solid and glucose recovery levels were 38.6% and 74.1%, respectively. Pretreated EFB were hydrolyzed and fermented with 40 FPU/g cellulose and 5% S. cerevisiae through the SHF, resulting in an 83.6% ethanol conversion rate. In order to purify the ethanol, distillation and dehydration facilities were operated. Distillation and dehydration efficiencies were 98.9% and 99.2%, respectively. The material balance was calculated using the results obtained from the operation of the pilot-scale bioethanol plant. In the pilot-scale plant, 144.4 kg of anhydrous ethanol (99.7 wt%) could be produced from 1000 kg dry weight of EFB. This result constitutes a significant contribution to the feasibility of bioethanol production from lignocellulosic biomass and justifies the scale-up to a commercial-scale plant.