The objective of this research has been to determine the impact of soil contamination with fluorine, including the concomitant application of substances neutralizing this pollution, on counts of actinomyces, fungi, and copiotrophic bacteria, and on the activity of acid phosphatase, alkaline phosphatase, grease, and dehydrogenase. The research was based on two pot experiments, both conducted in 2009 in a greenhouse at the University of Warmia and Mazury in Olsztyn, Poland.