Metaphase chromosome preparations were analyzed for structural aberrations and mitotic index was calculated parallely. The results are summarized in Tables 3–5.
The positive controls studied in this assay were found to induce statistically significant number of structural aberrations (Fig. 3).
Treatment of CHO-K1 cells with KalmColdTM both in the absence and presence of metabolic activation for 4 h and 16 h did not induce significant number of chromosome aberrations when compared with the solvent control.
Treatment of the cells with KalmColdTM significantly decreased the mitotic index values at the highest concentration tested in the study.