Based on our analyses, pitaya (H. polyrhizus) peel can be considered an important candidate for the production of novel amylase enzyme. The enzyme is calcium-independent and thermo-tolerant with activity in the high pH ranges. In addition, the amylase displayed proper stability in the presence of chelating and oxidising agents. These features, along with the rich and cost-effective source, indicate that this enzyme has potential as a useful amylolytic enzyme in various industrial applications, especially in the food industry.