Dried Krueo Ma Noy leaf powder was extracted under
conditions of varied pH (3–7) and temperature (25–90 8C)
with distilled water (Table 1) and it was found that the most
effective and economic condition was at room temperature
(25–28 8C) and natural pH (3.8–4.0) with a solid:water ratio
of 1:50 for 60 min. The crude extract was filtered through a
vacuum filter and centrifuged at 10,000 rpm for 15 min. The
supernatant was concentrated to about half of its volume in a
rotary evaporator before being precipitated with ethanol
95% (w/v) (final ethanol concentration was w70%). The
precipitate was dried in a vacuum oven and ground to
obtained crude extract powder