Fermentation sugar production from jackfruit seeds starch by 3 fungal strains was examined. Then the fermentable sugars produced so were tested as substrates for fermentation by S. cerevisiae YRK 017 to produce ethanol. The results showed that A. rouxii was the most effective organism for producing reducing sugar. Ethanol production process was studied on separated hydrolysis and fermentation (SHF) process and simultaneous saccharification and fermentation (SSF) process. The jackfruit seeds starch fermented by SHF process produced higher yield of ethanol than that from SSF process.