A cross-sectional study design was applied on 273 nurses to investigate associations of physical job demands, age, and musculoskeletal disorders (MSDs) in nursing. Concurrently, participants reported on various physical job demands with a standardized questionnaire for Hospital Work. As a special contribution,this investigation illustrates findings on musculoskeletal disorders provided by a standardized physical examination to questionnaire data. musculoskeletal disorders located in the lower back (8.7%) had the highest frequency, followed by the neck (7.3%), the shoulders (6.9%), and the knees (2.2%). There were significant differences in the frequencies of MSD between the young/middle age-group and the old age-group in most locations, while the only significant difference between the young and the middle age-group was found for shoulder- musculoskeletal disorders. Furthermore high levels of physical job demands increased the risk of musculoskeletal disorders significantly (OR = 5.7, 1.55-20.96) in all age-groups. The study provides further indication for development of ageadapted preventive measures.