The tissues
were then washed ten times with sterile water, cut into small pieces and homogenized in a blender
containing 90 ml sterile distilled water. Macerated tissues were diluted into 10-1 dilution by adding 9
volumes of sterile distilled water. Serial dilution was made up to 10-5 dilution by taking 1 ml of wellshaken
suspension and adding into 9 ml water blank tubes. 100