The population of L. acidophilus and S. boulardii in the synbiotic and probiotic ice cream were above the therapeutic level during processing and storage. It is documented that consumption of synbiotic and probiotic ice cream could significantly increase the gut flora and thereby improve the health of consumers. It is also found that, combination of L. acidophilus and S. boulardii rather than either alone had a much pronounced effect on the gut of human volunteers by increasing the count. Hence, it is concluded that ice cream can effectively be used as a medium to deliver probiotic bacteria and yeast as well as prebiotic substance like FOS to enhance the gut health of human beings.