In Mexico, the oil seed from Calophyllum brasiliense is traditionally used for lighting, but the oil seeds and
leaves have not yet been evaluated. In this work,two sites of C. brasiliense were studied: San Andres Tuxtla
andPajapan. The aimofthis study was to characterize the fatty acidprofile ofthe seedoil,leaves, andcallus
cultures from leaves or seed explants. Fatty acids of the leaves, seeds, and callus culture samples were
extracted with hexane, esterified, and then analyzed in a Gas chromatography–mass spectrometry. The
San Andrés Tuxtla seeds had a significantly higher (P ≤ 0.05) oil content: 58.2% versus 47.6% in Pajapan
seeds. The seed oil recovered from the San Andrés Tuxtla samples showed higher amounts of linoleic
acid (41.3%), while the oil recovered from the Pajapan seeds had remarkable oleic acid content (39.3%).
The physicochemical parameters of San Andrés Tuxtla and Pajapan oils such as the density, viscosity,
iodine, acid, and saponification values were measured and met the industrial standards for oil feedstock
for biodiesel. The biomass growth of callus cultures was enhanced, and callus could produce fatty acids.
These were mainly total saturated acids such as palmitic acid (31.32–39.18%) followed by stearic acid
(19.52–21.18%). The main unsaturated fatty acid was oleic acid with values ranging from 23.14 to 31.63%.
This is the first report on the fatty acid composition of seeds, leaves, and callus cultures from C. brasiliense.
Therefore, this study can help to promote the importance of this agricultural product and can lead to
biotechnological tools for the sustainable production of fatty acids or secondary metabolites.