Following assembly and
translation, Blast2GO analysis was performed on 3520 isotig
sequences against the UniProt database (Fig.3A). Of the total
number of isotigs obtained, 43% did not show similarity to any other
protein in the database, 3% were similar to predicted/hypothetical
proteins and 54% showed significant similarity to annotated
sequences (Fig.3A, left). The annotated sequences were grouped
according to their predicted biological function, including a group
of 16 sequences that were annotated as proteins from the hemocyanin
family, with different sequencing coverage (Fig.3A, right). Of the
16 hemocyanin-family isotigs, 12 were identified as hemocyanin,
three as C. quadricarinatus cryptocyanin (CqCc) and one as C.
quadricarinatus prophenoloxidase (CqPPO) (Fig.3B). Four of the
12 hemocyanin isotigs were found to be identical and were,