DPPH scavenging capacity Preparation of DPPH solution were adopted from Blois [9] with minor modification. Each extracts 50 μg/mL was pipetted into DPPH solution concentration 50 μg/mL (1:1) to initiate the reaction. After 30 minutes incubation, the absorbance was read at wavelength 516 nm by using spectrophotometer UV-Vis Hewlett Packard 8435. Methanol was used as a blank and DPPH solution 50 g/mL as standard. Analysis was done in triplicate for standard and each extracts. Antioxidant activity of each extracts were determined based on the reduction of DPPH absorbance by calculating percentage of antioxidant activity [8].