Larvae of 3rd and 4th instars of A. aegypti colonies were used for the bioassays. The mosquitoes maintained in the laboratory were fed 0.2 g/100 mL puppy food (Purina Cat Chow®). All bioassays were performed at 25±2 ◦C temperature and 70–85% RH in an ELETROlab® 132FC incubator, with photoperiod of 14:10 light–dark cycles.