In the present study, an attempt was made to generate
plants using somatic cell culture approach from two
genotypes that is, FR13A and FR43B. The experiment
was successful as plantlets could be generated from the
callus developed from the mature seeds. Experiment was
also undertaken by exposing the somaclones to different
level of gamma radiation. The callus induction frequency
was optimum and the production of plantlets from the
mutagen treated grains was successful at both 20K and
25 Kr radiation dose. The callus induction frequency was
relatively higher in FR43B than in FR13A. There was a
steady increase in the callus induction potential with
increase in radiation dose from 20 - 25 Kr. However,
there was no plant germination at 30 Kr in both the genotypes.
This may be due to the development of deleterious
mutations at high dose of gamma radiation (Cheema and
Atta, 2003).