To detect polymorphisms in the CDC10 gene, we used two steers with the highest and two steers with the lowest performance for CWT from the 27 JB steers that were selected for real-time PCR and sequenced ~4 kb proximal to the promoter region of the CDC10 gene. One SNP, NC_007302.5:g.63264949G>C, was detected in the promoter region of the CDC10 gene and genotyped by
PCR-RFLP for genetic diversity and association analyses (Table S2).