The ethylene producing capacity of the discs was determined with and without
the application of I-aminocyclopropane-1-carboxylic acid (ACC) 16 hours after
treatment. The ACC was applied as 30 ~1 of a 5 mM solution directly to each disc.
Discs were sealed for one hour in the 25ml conical flasks in which they were
treated using Suba-Seals. The headspace was then analyzed for ethylene by gas chromatography.