There for, the optimum combination of the variables (initial pH, KH2 PO4 , Ca2+ and incubation temperature) which had the highest significant influence on inulinase production was further analyzed by a Taguchi method for the best solid state fermentation bringing maximum inulinase activity. Other variables with less significant effect were not included in the next optimization experiment, but instead were used in all trials at their (−1) level and (+1) level, for the negatively contributing variables and the positively contribut-ing variables, respectively.