2.8. In vitro release studies
In vitro release studies were conducted at pH 6.8 (PBS). For this,0.1 g dried microcapsules were weighted and put into a dialysisbag (cutoff molecular weight: 14 kDa). Then 10 ml PBS (pH 6.8)solution was added. The dialysis bag was immersed in a 200 mlmedium solution in a beaker. The beaker was mixed consistentlyat 100 rpm at room temperature, and 5 ml of the medium was takenfrom the beaker at time intervals, with the removed volume beingreplaced with new medium. The concentration of the coriander oilreleased to the medium was calculated according to the formulagiven above at 538 nm in a UV–vis spectrophotometer. To calculatethe cumulative release percentage (CRP), the following formula wasused:
CRP= tt = 0MtM0 ×100 (4)Where, M0represents the essential oil amount at the beginningand Mtrepresents the essential oil amount at the current time. Theanalyses were repeated three times.