%Recovery ¼ Observed concentration
Fortification level 100
The standard curve was established by using the ratio of the
peak area of the analyte and the internal standard (Y) and the ratio
of the concentration of the analyte and the internal standard (X).
LOD and LOQ were defined, respectively, as the signal corresponding
to 3 and 10 times the noise ratio, determined experimentally
from fortified samples (European Commission (EC)
SANCO/2013/12571), obtained from peak areas of 10 independent