The sterilization test was conducted in accordance with the
constant-temperature test and the microbacterial test for retort
food stipulated in the Food Sanitation Act. The test was done five
times. The ohmically heated samples, held at 35 °C for 14 days,
were cooled to 20 °C before their morphology was observed. The
samples whose packaging containers were inflated or from which
there was content leakage were considered microorganism-positive.