2.4.2. Radical scavenging activities on DPPH
The free radical scavenging activity of the essential oil was
measured using DPPH by the method of Blois (Blois, 1958). A
0.1 mM solution of DPPH in methanol was prepared and 1 mL of
this solution was added to 3 mL of various concentrations
(0.2e1.0 mg/mL) of sample dissolved in methanol to be tested. After
30 min, absorbance was measured at 517 nm. Vc was used as
a reference material. All tests were performed in triplicate. The
scavenging activity was calculated as follows: