The optimal activity temperature of isolated amylase was 40°C and the activity decreased dramatically at temperatures above 42°C. Amylase activity occurred at pH 6.0-9.5 with a maximum at pH 8.0. Starch degradation by amylase was analyzed through HPLC and FTIR analysis. The results from the study suggest that amylase from Bacillus megaterium may be suitable in liquefaction of starch in high temperature and alkaline condition in detergent, textile, food and other industrial applications