Rice bran protein was extracted by dispersing milled defatted rice bran in distilled water
(mentioned at above) at a ratio of 1:4 (w/v) at pH 9.5 and agitated all the time during extraction,
then, centrifuged at 15,200 xg at 25 °C for 30 min. The supernatant obtained was rice bran protein
extract (RBPE). Using the general procedure described above, the extracting conditions, including