Microorganisms
Two starter culture strains (Lactobacillus
sakei BCC 102 and Debaryomyces hansenii BCC
106) isolated from Nham were chosen after
screening for nondecarboxylase activities. Both
strains were gift from the Culture Collection
Laboratory, National Center for Genetic
Engineering and Biotechnology (BIOTEC),
Patumthani, Thailand. Cultures were stored at -
80°C in 20% glycerol. One loopful of a stock
culture was cross-streaked on Man, Rogosa and
Sharpe (MRS) agar and then incubated at 30°C
for 48 h. A single colony on MRS agar was grown
in MRS broth at 30°C for 18-24 h.