2. Materials and methods
2.5.1. Instrument conditions
Samples were separated by a C-18 column. Mobile phase used was a combina- tion of deionised water with 0.1 g/l KBr, Methanol and Acetonitrile in the ratio
63:22:15 with a flow rate of 1.2 ml/min. The detection and quantification were per- formed with an excitation-365 nm, emission-464 nm and Kobra cell at 100 l-amps using a fluorescence detector (RF-10AXL Shimadzu Liquid Chromatogaphy). The
retention time of the sample was compared with standard aflatoxin (Supelco). Afla- toxin was calculated from the formula:
pG ðconcÞx 1 x 1000 x 50 x 100 x 1
2.1. Extraction of essential oil