The experimental analysis of the nucleotide phosphodiesterase at concentrations of 0.01 to 0.03%, the amount of enzyme that is added to make the nucleotide it. will increase accordingly It also found that increasing the amount of time to react. Nucleotide was increased by 14 and 16 hr., by which time the amount nucleotide not differ significantly. Thus, the optimal conditions for producing nucleotide is the concentration of the enzyme phosphodiesterase 0.03 % to the nucleotide equal to 3.76 % and time to react 14 hr. The amount nucleotide equals 5.25 %