The qualitative methods have been widely used for the
screening potential of the lipolytic activity. In this study,
samples were screened using Tween 20 agar plate,
Rhodamine B and phenol red with in 3 days incubation, the
microorganism showed lipolytic activity. Screening using
Tween 20 agar plates showed precipitated around the
organism. From the result in Table IV, there are two areas
was positive has lipolytic activity (B and C). Agar plates
containing olive oil and Rhodamine B appear opaque and are
pink colored. After incubation sample was monitored by
irradiating plate with UV light at 350 nm. Microbial colonies
began to show an orange fluorescence and continuing
incubation time orange fluorescent halos were formed around
the colonies. From the result in Table III, was shown that all
of sample area was positive has lipolytic activity.