The primers
named FIP (composed of F1c and F2) and BIP (composed
of B1c and B2) were the inner primer set; F3 and B3 were
the outer primer set [12]. The respective sites for these
primers are shown in Fig. 1. The predicted size from F3
to B3c site for 18S rRNA and HBB were 197 and 192 bp,
respectively.