3.1. Effect of triazophos on the protein content in the fat bodies and ovaries of N. lugens adult females under eCO2
The three-way ANOVA of the data (Fig. 1), showed that insecticide concentration, rice variety and CO2 concentrations significantly influenced the protein content of fat bodies in the adult females (Table 1). The protein content of fat bodies significantly increased with 40 ppm triazophos treatment for both MH63 and TT51 varieties at different DAEs, under both aCO2 or eCO2 (Fig. 1A–C). For the two concentrations of CO2, the grand mean (means of main effect) of the protein content of fat bodies treated with 40 ppm triazophos under eCO2 was significantly higher than under aCO2, increasing by 45.4% and 47.4%, at 1 and 3 DAE, respectively. For the two rice varieties, the grand mean of the protein content of fat bodies in TT51 treated with 40 ppm triazophos was significantly higher than that in MH63, increasing by 38.9% and 29.6%, at 1 and 3 DAE, respectively. Multiple comparisons of the means indicated that the protein content was significantly higher in the fat bodies treated with 40 ppm triazophos for both MH63 and TT51 than in the untreated controls; the protein content was significantly higher in the fat bodies for both MH63 and TT51 under eCO2 than in those under aCO2.
3.1. Effect of triazophos on the protein content in the fat bodies and ovaries of N. lugens adult females under eCO2The three-way ANOVA of the data (Fig. 1), showed that insecticide concentration, rice variety and CO2 concentrations significantly influenced the protein content of fat bodies in the adult females (Table 1). The protein content of fat bodies significantly increased with 40 ppm triazophos treatment for both MH63 and TT51 varieties at different DAEs, under both aCO2 or eCO2 (Fig. 1A–C). For the two concentrations of CO2, the grand mean (means of main effect) of the protein content of fat bodies treated with 40 ppm triazophos under eCO2 was significantly higher than under aCO2, increasing by 45.4% and 47.4%, at 1 and 3 DAE, respectively. For the two rice varieties, the grand mean of the protein content of fat bodies in TT51 treated with 40 ppm triazophos was significantly higher than that in MH63, increasing by 38.9% and 29.6%, at 1 and 3 DAE, respectively. Multiple comparisons of the means indicated that the protein content was significantly higher in the fat bodies treated with 40 ppm triazophos for both MH63 and TT51 than in the untreated controls; the protein content was significantly higher in the fat bodies for both MH63 and TT51 under eCO2 than in those under aCO2.
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